发表在免疫杂志October 2013,的文章Spatiotemporal Dynamics of Intratumoral Immune Cells Reveal the Immune Landscape in Human Cancer 根据公共数据库的 28 cell types ，定下来了 Five hundred seventy-seven cell-type-specific genes (681 Affymetrix probes) 。
然后根据这 28 cell types 的基因可以把CRC分成两类，如下：

We then performed microarray expression experiments and analyzed the 681 probes in tumors from 105 CRC patients and five histologically normal colon mucosa. Two clusters of patients were found, and 372 probes were significantly differentially expressed between the two groups (Figure 1B).
两组病人高表达的基因分别是：

• An overrepresentation of highly expressed genes specific for T cell populations and subpopulations of Th1, Tγδ, cytotoxic T cells as well as for macrophages and mast cells was significantly associated with the patient cluster 1 (all p values < 0.05).
• In comparison, patient cluster 2 showed an overrepresentation of highly expressed genes specific for eosinophil, Tcm, Th2, Th17, Treg, and NK cell subpopulations (all p values < 0.05).
  他们两组病人有统计学显著的生存差异：
  
  而且不仅仅是表达芯片数据，后来还引入了qPCR数据和TMA数据。
  这577个基因，在文章附件表格里面有。