看到发表在19 July 2019的文章Strand-specific miR-28-3p and miR-28-5p have differential effects on nasopharyngeal cancer cells proliferation, apoptosis, migration and invasion,全文提供实验验证了miR-28-3p and miR-28-5p的生物学功能不一样。
但是我们作为数据分析人员,很难有这么复杂的背景知识,有时候仅仅是把表达矩阵给过滤,大家都懂的过滤操作。
In some cases, two mature miRNAs can be excised from the same stem-loop pre-miRNA
These “5p” and “3p” miRNAs are biologically different in terms of stability and functionality. In humans, two mature miRNAs, miR-28-3p and miR-28-5p, are derived from 3′ and 5′ ends of pre-miR-28, respectively. miR-28-3p and miR-28-5p targets several cancer-related genes and is hence involved in cell proliferation, migration, invasion and epithelial–mesenchymal transition (EMT)